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Figure 6. Metformin and CAF-1. (A) Western blots for CAF-1 major subunits, <t>p150</t> and p60, of CAL27 treated with metformin for 24, 48 and 72 hours. (B) CAF-1 silencing by siRNAs in CAL27 assessed via WB. (C) Wound healing assay of CAL27 silenced for CAF-1/p150 and CAF-1/p60. (D) Invasion assay of CAF-1 silenced CAL27. (E) Immunofluorescence of E- and N-cadherin of CAL27 silenced for CAF-1 major subunits (scale bar = 20 μm). (F) Western blots for CAF-1 major subunits, p150 and p60, of SCC154 treated with metformin for 24, 48 and 72 hours. (G) CAF-1 silencing by siRNAs in SCC154 assessed via WB. (H) Wound healing assay of SCC154 silenced for CAF-1/p150 and CAF-1/p60. (I) Invasion assay of CAF-1 silenced SCC154. (J) Immunofluorescence of E- and N-cadherin of SCC154 silenced for CAF-1 major subunits (scale bar = 20 μm). Significant differences from control conditions are indicated by asterisks (* p ≤ 0,05; ** p ≤ 0,01).
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Figure 6. Metformin and CAF-1. (A) Western blots for CAF-1 major subunits, p150 and p60, of CAL27 treated with metformin for 24, 48 and 72 hours. (B) CAF-1 silencing by siRNAs in CAL27 assessed via WB. (C) Wound healing assay of CAL27 silenced for CAF-1/p150 and CAF-1/p60. (D) Invasion assay of CAF-1 silenced CAL27. (E) Immunofluorescence of E- and N-cadherin of CAL27 silenced for CAF-1 major subunits (scale bar = 20 μm). (F) Western blots for CAF-1 major subunits, p150 and p60, of SCC154 treated with metformin for 24, 48 and 72 hours. (G) CAF-1 silencing by siRNAs in SCC154 assessed via WB. (H) Wound healing assay of SCC154 silenced for CAF-1/p150 and CAF-1/p60. (I) Invasion assay of CAF-1 silenced SCC154. (J) Immunofluorescence of E- and N-cadherin of SCC154 silenced for CAF-1 major subunits (scale bar = 20 μm). Significant differences from control conditions are indicated by asterisks (* p ≤ 0,05; ** p ≤ 0,01).

Journal: Pathologica

Article Title: Metformin radiosensitizes OSCC in 2D and 3D models: possible involvement of CAF-1

doi: 10.32074/1591-951x-1085

Figure Lengend Snippet: Figure 6. Metformin and CAF-1. (A) Western blots for CAF-1 major subunits, p150 and p60, of CAL27 treated with metformin for 24, 48 and 72 hours. (B) CAF-1 silencing by siRNAs in CAL27 assessed via WB. (C) Wound healing assay of CAL27 silenced for CAF-1/p150 and CAF-1/p60. (D) Invasion assay of CAF-1 silenced CAL27. (E) Immunofluorescence of E- and N-cadherin of CAL27 silenced for CAF-1 major subunits (scale bar = 20 μm). (F) Western blots for CAF-1 major subunits, p150 and p60, of SCC154 treated with metformin for 24, 48 and 72 hours. (G) CAF-1 silencing by siRNAs in SCC154 assessed via WB. (H) Wound healing assay of SCC154 silenced for CAF-1/p150 and CAF-1/p60. (I) Invasion assay of CAF-1 silenced SCC154. (J) Immunofluorescence of E- and N-cadherin of SCC154 silenced for CAF-1 major subunits (scale bar = 20 μm). Significant differences from control conditions are indicated by asterisks (* p ≤ 0,05; ** p ≤ 0,01).

Article Snippet: Primary antibodies anti-CHAF1B (rabbit polyclonal HPA021679; Sigma-Aldrich, St. Louis, MO, USA); CAF-1 p150 (mouse monoclonal sc-32742; Santa Cruz, Dallas, TX, USA); GAPDH (rabbit monoclonal, (14C10) #2118; Cell Signaling Technology, Danvers, MA, USA) were used overnight at 4°C.

Techniques: Western Blot, Wound Healing Assay, Invasion Assay, Immunofluorescence, Control